For the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype.

N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation (1). This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). It can be used as a substitute for the N-1 Bottenstein formulation. N-2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines. (in Current Methods in Cellular Neurobiology: Volume 4, edited by Jeffery Barker, John Wiley & Sons, Inc.,1983.)

COMPONENTS	Molecular Weight	Concentration (mg/L)	mM
Proteins
Human Transferrin (Holo)	10000	10000	1
Insulin Recombinant Full Chain	5807.7	500	0.0861
Other Components
Progesterone	314.47	0.63	0.002
Putrescine	161	1611	10.01
Selenite	173	0.52	0.00301

                                         
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